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Primary antibodies used for Western blotting and immunohistochemistry analyses.

Journal: Frontiers in Pharmacology

Article Title: Isoalantolactone exhibits activity against Echinococcus multilocularis associated with mitochondrial perturbation and changes in the lesion microenvironment

doi: 10.3389/fphar.2026.1949982

Figure Lengend Snippet: Primary antibodies used for Western blotting and immunohistochemistry analyses.

Article Snippet: RUNX2 , ABclonal, Wuhan, China , A2851SP , 1:1,000 , —.

Techniques: Western Blot, Immunohistochemistry

Treatment-associated protein-expression changes in the AE lesion microenvironment. (A) Ultrastructural changes of metacestodes observed by TEM in the untreated, ABZ (100 mg/kg), ISO 10 mg/kg, and ISO 40 mg/kg groups. Representative ultrastructural alterations included mitochondrial swelling and disruption of mitochondrial cristae, cytoplasmic vacuolization, dilated rough endoplasmic reticulum, and autophagosome-like structures. Labels indicate primary lysosomes (PLY), mitochondria (Mi), nuclei (N), collagen fibers (CF), autophagosome-like structures (Auto), and rough endoplasmic reticulum (RER). (B) Expression and quantitative analysis of apoptosis-related proteins (cleaved caspase-3, Bcl-2, and Bax). (C) Expression and quantitative analysis of PCNA. (D) Expression and quantitative analysis of CD31. (E) Expression and quantitative analysis of RUNX2, SPP1, Col1a1, Col3a1, MMP2, and MMP9. ISO treatment was associated with higher RUNX2, SPP1, Col1a1, and Col3a1 expression and lower MMP2 and MMP9 expression. (F) TGF-β1 expression was lower in the ISO group than in the untreated group. p-Smad2/3 was also decreased, while total Smad2/3 showed no significant change. (G) Expression and quantitative analysis of TLR4/NF-κB pathway-related proteins (MyD88, NF-κB p65, p-NF-κB p65, TLR4).* Indicates p < 0.05. ** Indicates p < 0.01. *** Indicates p < 0.001. **** Indicates p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Isoalantolactone exhibits activity against Echinococcus multilocularis associated with mitochondrial perturbation and changes in the lesion microenvironment

doi: 10.3389/fphar.2026.1949982

Figure Lengend Snippet: Treatment-associated protein-expression changes in the AE lesion microenvironment. (A) Ultrastructural changes of metacestodes observed by TEM in the untreated, ABZ (100 mg/kg), ISO 10 mg/kg, and ISO 40 mg/kg groups. Representative ultrastructural alterations included mitochondrial swelling and disruption of mitochondrial cristae, cytoplasmic vacuolization, dilated rough endoplasmic reticulum, and autophagosome-like structures. Labels indicate primary lysosomes (PLY), mitochondria (Mi), nuclei (N), collagen fibers (CF), autophagosome-like structures (Auto), and rough endoplasmic reticulum (RER). (B) Expression and quantitative analysis of apoptosis-related proteins (cleaved caspase-3, Bcl-2, and Bax). (C) Expression and quantitative analysis of PCNA. (D) Expression and quantitative analysis of CD31. (E) Expression and quantitative analysis of RUNX2, SPP1, Col1a1, Col3a1, MMP2, and MMP9. ISO treatment was associated with higher RUNX2, SPP1, Col1a1, and Col3a1 expression and lower MMP2 and MMP9 expression. (F) TGF-β1 expression was lower in the ISO group than in the untreated group. p-Smad2/3 was also decreased, while total Smad2/3 showed no significant change. (G) Expression and quantitative analysis of TLR4/NF-κB pathway-related proteins (MyD88, NF-κB p65, p-NF-κB p65, TLR4).* Indicates p < 0.05. ** Indicates p < 0.01. *** Indicates p < 0.001. **** Indicates p < 0.0001.

Article Snippet: RUNX2 , ABclonal, Wuhan, China , A2851SP , 1:1,000 , —.

Techniques: Expressing, Disruption, Analysis